DNA Methylation Sequencing

DNA methylation is essential for numerous developmental processes, gene silencing and cancer progression. DNA methylation sequencing allows researchers to study and identify regions throughout the genome which epigenetically regulate gene expression. 

Introduction 

DNA methylation is essential for numerous developmental processes, gene silencing and cancer progression. DNA methylation sequencing allows researchers to study and identify regions throughout the genome which epigenetically regulate gene expression. 

To identify and track these regions, genomic DNA is first bisulfite-treated, converting all un-methylated cytosines to uracils. 

During PCR amplification, uracil is then converted to thymidine while methylated cytosine remains unaffected. Subsequent sequencing of both treated and un-treated DNA will reveal methylated regions of the DNA. Utilizing paired-end sequencing to accurately map reads to the reference sequence and generate large-scale, high resolution genomic DNA methylation profiles. Ambry Genetics has years of experience generating various types of methylation sequencing libraries and targeted enrichment products.